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Spectrum manufactures a complete range of Ion Exchange Resins for preparative scale ion
exchange chromatography. Spectra/Gel® Ion Exchange Resins are ideally suited for protein
purification, antibody isolation, and peptide fractionation. They are also used in
traditional inorganic ion exchange.
Spectra/Gel Ion Exchange Resins are durable, insoluble, and compatible with most aqueous and
organic solvents. The structural polymeric backbone of the resin is styrene cross-linked with 2 to
8% divinylbenzene. The amount of cross-linkage determines both the pore size of the media and the capacity
of the resin. (The pore size can be significant when separating proteins but is of little significance when doing
inorganic separations.)
The type of exchange performed (cation or anion) is determined by functional groups added to the resins.
The resins are available in 3 different bead sizes. The medium (75-150 µm diameter) and
large (150-300 µm diameter) beads are most useful for gravity flow and batch (non-flow)
applications. The larger size of the beads makes for ready flow and easy separation from a bulk liquid.
The medium (75-150 µm diameter) and small (40-75 µm diameter) beads are most useful
for pumped applications. The smaller size of the beads reduces the chromatography plate height providing a
more rapid equilibration at higher flow rates.
These resins are available with 3 different levels of cross-linkage in the polystyrene backbone.
The greater the cross-linkage the greater the capacity of the resin and the smaller the pore size.
For inorganic ion exchange the pore size is usually of little interest. For globular proteins, the approximate
exclusion limits are 3000 Daltons at 2%, 1500 Daltons at 4% and 1000 Daltons at 8% cross-linkage.
Spectra/Gel Anion Exchange Resins are strong (type 1) ion exchange resins. They use a
trimethylbenzylammonium group as the exchange site. They are supplied in the chloride form (with
a chloride ion occupying the exchange site). This form is very stable and has an excellent shelf life.
Anion Exchange Resin Characteristics
| Type: | strong base anion exchanger |
| Active Group: | trimethylbenzylammonium |
| % divinylbenzene: | 2%, 4%, or 8% |
| Supplied Ionic Form: | Cl- |
| Moisture Content: | 43% to 48% |
| Volume Change: | Cl- to OH- is +20% |
| pH Range: | 0 to 14 |
| Selectivity: | Cl-/OH- is about 25 |
| Order of Selectivity: | I > NO3 > Br > Cl > acetate > OH > F |
Ordering Information: click on Product No. to add to cart
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| Product No. | Description | Bead Size | Capacity | DVB | Qty | Price |
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| 122045 | IE 1X2 Resin | 40 to 75 µm (200 to 400 mesh) | 0.6 meq/ml | 2% | 0.5 kg | $275 |  |
| 122050 | IE 1X2 Resin | 75 to 150 µm (100 to 200 mesh) | 0.6 meq/ml | 2% | 0.5 kg | $275 | |
| 122055 | IE 1X2 Resin | 150 to 300 µm (50 to 100 mesh) | 0.6 meq/ml | 2% | 0.5 kg | $275 | |
| 122060 | IE 1X4 Resin | 40 to 75 µm (200 to 400 mesh) | 1.0 meq/ml | 4% | 0.5 kg | $275 | |
| 122070 | IE 1X4 Resin | 150 to 300 µm (50 to 100 mesh) | 1.0 meq/ml | 4% | 0.5 kg | $275 | |
| 122075 | IE 1X8 Resin | 40 to 75 µm (200 to 400 mesh) | 1.2 meq/ml | 8% | 0.5 kg | $275 | |
| 122080 | IE 1X8 Resin | 75 to 150 µm (100 to 200 mesh) | 1.2 meq/ml | 8% | 0.5 kg | $275 | |
| 122085 | IE 1X8 Resin | 150 to 300 µm (50 to 100 mesh) | 1.2 meq/ml | 8% | 0.5 kg | $275 | |
Spectra/Gel Cation Exchange Resins are strong acid (type 50) ion exchange resins. They contain a
sulfonic acid active group as the exchange site. They are supplied with a hydrogen ion occupying the exchange site.
Cation Exchange Resin Characteristics
| Type: | strong acid cation exchanger |
| Active Group: | sulfonic acid |
| % divinylbenzene: | 2%, 4%, or 8% |
| Supplied Ionic Form: | H+ |
| Moisture Content: | 51% to 54% |
| Volume Change: | Na+ to H+ is +8% |
| pH Range: | 0 to 14 |
| Selectivity: | Na+/H+ is about 1.5 |
| Order of Selectivity: | Ba++ > Rb++ > Ca++ > Mg++ > Be++ > Ag+ > Cs+ > Rb+ > K+ > NH4+ > Na+ > H+ > Li+ |
Ordering Information: click on Product No. to add to cart
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| Product No. | Description | Bead Size | Capacity | DVB | Qty | Price |
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| 122000 | IE 50X2 Resin | 50 to 75 µm (200 to 400 mesh) | 0.6 meq/ml | 2% | 0.5 kg | $275 | |
| 122005 | IE 50X2 Resin | 75 to 150 µm (100 to 200 mesh) | 0.6 meq/ml | 2% | 0.5 kg | $275 | |
| 122010 | IE 50X2 Resin | 150 to 300 µm (50 to 100 mesh) | 0.6 meq/ml | 2% | 0.5 kg | $275 | |
| 122015 | IE 50X4 Resin | 40 to 75 µm (200 to 400 mesh) | 1.2 meq/ml | 4% | 0.5 kg | $275 | |
| 122025 | IE 50X4 Resin | 150 to 300 µm (50 to 100 mesh) | 1.2 meq/ml | 4% | 0.5 kg | $275 | |
| 122030 | IE 50X8 Resin | 40 to 75 µm (200 to 400 mesh) | 1.7 meq/ml | 8% | 0.5 kg | $275 | |
| 122035 | IE 50X8 Resin | 75 to 150 µm (100 to 200 mesh) | 1.7 meq/ml | 8% | 0.5 kg | $275 | |
| 122040 | IE 50X8 Resin | 150 to 300 µm (50 to 100 mesh) | 1.7 meq/ml | 8% | 0.5 kg | $275 | |
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