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The detector is a necessary part of any experimental chromatography system. It is
used to determine the concentrations of the different compounds as they elute from
the column. It also provides information on the condition of the column and on the
identity of the sample components.
The output of the detector is connected to a strip chart recorder or data system.
The recorded trace becomes the chromatogram describing the separation.
The detector output can also be connected to an intelligent fraction collector
(like the CF-1)
to provide real-time peak detection and allow you to control how the components
of your precious samples are collected.
The Spectra/Chrom® Model 280 UV detector is a fixed wavelength flow-through
spectrophotometer ideally suited for use as a chromatographic detector. It works
equally well with HPLC and low-pressure systems. It features a direct reading
digital output and a dual beam optical system that allows the automatic subtraction
of a solvent background even in the presence of a rapidly changing gradient.
It has analog outputs for both a chart recorder and an integrator (1V/AU).
As with all of Spectrum's chromatography instruments, the Model 280 UV detector
comes complete and ready-to-use. It includes a 5 mm pathlength flow cell, filters
for both 254 and 280 nm operation, and a set of tubing fittings. Unlike many filter
based detector, the operating wavelength can be changed in less than 10 seconds.
Special Features of the Model 280 Include:
- Dual-Beam optics for baseline subtraction
- Cold-room compatible
- Digital Readout
- Detection limit 3 x 10-4 AU
- Both 254 and 280 nm operation
- 3 different flow cells available
- Quick wavelength changes
- CE marked & complies with RoHS
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| Full-Scale Absorbance Ranges: | 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, and 1.0 Absorbance Units |
| Linearity: | Maximum error 1.5% of full scale at 254 nm |
| Noise Level: | less than 0.0003 AU at 254 nm with dry flow paths after 30 minute warm-up |
| Stability: | Maximum drift 0.0005 AU per hour after 30 minute warm-up |
| Rise Time: | 10-90% rise time is 0.6 sec on 0.5 AU range |
| Display Accuracy: | +/- 3 counts |
| Dimensions: | 9.25 x 4.75 x 8 inch
23.5 x 12 x 20.3 cm
w x h x l |
| Weight: | 4.1 kg (9 lb) |
| Operating Temperature: | 0°C to 40°C, noncondensing |
| Power Requirements: | 115 ± 20 VAC
230 ± 40 VAC
20 VA |
| Line Frequency: | 45 to 65 Hz |
Ordering Information: click on Product No. to add to cart
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| Product No. | Description | Price |
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| 142600 | Model 280 UV detector, 115V, includes 5 mm pathlength flow cell and filters for 254 and 280 nm. | $2,558 |  |
| 142602 | Model 280 YV detector, 230V, includes 5 mm pathlength flow cell and filters for 254 and 280 nm. CE & RoHS. | $2,665 | |
| 142608 | 254/280 nm Filter slide. (This combination slide is included with the Spectra/Chrom UV Monitor.) | $439 | |
| 142642 | 5 mm pathlength dual flow cell. (This flow cell is included with Spectra/Chrom UV Monitor.) Illuminated volume 34 µL; hold-up volume 134 µL (per flow path). | $368 | |
| 142644 | 2 mm pathlength dual flow cell. Illuminated volume 14 µL; hold-up volume 54 µL (per flow path). | $428 | |
| 142640 | 10 mm pathlength dual flow cell. Illuminated volume 68 µL; hold-up volume 268 µL (per flow path). | $428 | |
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