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Equilibrium Dialysis


Binding Studies Under Controlled Dialysis

Combining efficiency, convenience, precision & reproducibility; Spectrum's Equilibrium Dialyzers are specifically designed to perform complex binding studies essential in biochemistry and pharmacology. Up to 20 independent studies are conducted simultaneously under the same controlled environmental conditions. Each non-binding Teflon reaction cell contains a dialysis membrane disc through which ligand freely passes from one half-cell and irreversibly binds to macromolecules trapped in the second half-cell. There are 3 reaction cell sizes available (Micro Cell, Semi-micro Cell & Macro Cell) each providing a different half-cell volume for the reaction solutions. Cell carriers containing 5 loaded and stacked cells are submerged in a water bath and rotated continuously to maximize binding efficiency and minimize concentration polarization. By operating the Equilibrium Dialyzer in a cold room or with heated water supply, binding studies can be conducted under an ideal temperature (-10 to 65 °C). There are two flat-sheet equilibrium dialysis systems available, depending on the preferred quantity of reaction cells.


5-Cell Equilibrium Dialyzer



This easy to operate flat-sheet dialysis system is designed to simultaneously conduct 5 different binding studies under the same environmental conditions. Locked into a base plate assembly, the cell carrier containing 5 loaded reaction cells is submerged in a water bath and rotated by a drive belt. The drive unit can be adjusted from 5 to 20 RPM to achieve optimum dialysis conditions while the water bath circulates for ultimate temperature control.

20-Cell Equilibrium Dialyzer



Designed to accommodate up to 20 independent binding reactions, the 20-Cell Equilibrium Dialyzer provides maximum results for minimal effort. This flat-sheet dialysis system with easy-to-carry recessed handles sits inside of an acrylic water bath that provides temperature control. Up to 4 cell carriers are rotated with a 5 to 30 RPM adjustable speed control. All components in direct contact with the water bath are built of corrosion resistant stainless steel and plastics.

Features & Benefits


  • Conducts 5 independent binding studies simultaneously
  • Continuous & speed controlled rotation ensures efficient reaction
  • Accommodates Micro, Semi-micro & Macro Cells for different volumes
  • Water bath can be circulated for ultimate temperature control

Features & Benefits


  • Conducts up to 20 independent binding studies simultaneously
  • Continuous & speed controlled rotation ensures efficient reaction
  • Accommodates Micro, Semi-micro & Macro Cells for different volumes
  • Water bath can be circulated for ultimate temperature control

Product Specifications

Dimensions:31 cm L x 10 cm W x 21.5 cm H
Power Supply:115V, 50/60 HZ
Net Weight:4.5 kg (10 lbs.)

Product Specifications

Dimensions:26 cm L x 19 cm W x 30 cm H
Power Supply:115V or 240V, 50/60 HZ
Net Weight:7.5 kg (16 lbs.)

Components


  • 5 to 20 RPM Drive Unit
  • 5 Micro, Semi-micro or Macro Cells
  • Acrylic Water Bath (5-cell size)
  • 50 S/P 4 Dialysis Membrane Discs (33 or 47 mm, 12-14 kD MWCO)
  • Base Plate Assembly
  • 1 Cell Carrier
  • 30 Stoppers
  • 6 Stainless Steel Spacers
  • 1 Blunt Nose Needle
  • 2 Flanges w/ 3 Knurled Nuts
  • Instruction Manual
  • Assembly Stand for Cell Carrier

Components


  • 5 to 30 RPM Drive Unit
  • 20 Micro, Semi-micro or Macro Cells
  • Acrylic Water Bath (20-cell size)
  • 50 S/P 4 Dialysis Membrane Discs (33 or 47 mm, 12-14 kD MWCO)
  • 4 Cell Carriers
  • 24 Stainless Steel Spacers
  • 120 Stoppers
  • 4 Sets of 2 Flanges with 3 Knurled Nuts Instruction Manual
  • 1 Blunt Nose Needle
  • Assembly Stand for Cell Carrier

Reaction Cell Specifications:

(for use with 5 & 20 Cell Equilibrium Dialyzer)
Cell TypeMicroSemi-microMacro
Half-cell Volume, Max0.25 ml1.36 ml6.0 ml
Half-cell Volume, Min*0.125 ml0.7 ml3.0 ml
Cell Diameter40 mm60 mm60 mm
Membrane Diameter33 mm47 mm47 mm
Membrane Surface Area2.0 cm24.5 cm211.0 cm2
Q-factor**166.43.5
Cell Spacer TypeMicroMacroMacro

* Fill cells to at least the cell center point to provide complete immersion of the membrane disc area during operation.
** Q-factor is the ratio between membrane surface area and half-cell working volume. The Q-factor should be as high as possible to reduce dialysis time. (Ex: 16 = 2.0 cm2 / 0.125 ml)

 

Ordering Information: Equilibrium Dialyzers click on Product No. to add to cart

Part No.DescriptionVoltageCell TypePrice (US$)

132460B20-Cell Equilibrium Dialyzer115 VMacroCell$14,159order part 132460B
132460Semi-Micro Cell$14,159order part 132460
132460MMicro Cell$14,159order part 132460M
132461B20-Cell Equilibrium Dialyzer240 V
CE Marked
MacroCell$14,452order part 132461B
132461Semi-Micro Cell$14,452order part 132461
132461MMicro Cell$14,452order part 132461M
132360B5-Cell Equilibrium Dialyzer115 VMacroCell$3,164order part 132360B
132360Semi-Micro Cell$3,164order part 132360
132360MMicro Cell$3,164order part 132360M

Ordering Information: Replacement Parts click on Part No. to add to cart

Part No.DescriptionQtyFor Use WithPrice (US$)

132341Macro CellSet of 55-Cell & 20-Cell Eq. Dialyzers$2,129order part 132341
132342Semi-Micro Cell$2,129order part 132342
132343Micro Cell$2,129order part 132343
132344Macro CellSingle Set$433order part 132344
132345Semi-Micro Cell$433order part 132345
132346Micro Cell$433order part 132346
132349Blunt Nose Needle, 22G1/pkgMacro, Semi-Micro & Micro Cells$67order part 132349
132350Black Stoppers30/pkg$93order part 132350
132347Temperature Spacer1/pkgMacro & Semi-Micro Cells$82order part 132347
132348Micro Cells$61order part 132348
132352Cell Carrier1/pkg20-Cell Eq. Dialyzer$502order part 132352
1323725-Cell Eq. Dialyzer$545order part 132372
132370Stand for Cell Carrier1/pkgCell Carriers$157order part 132370
132371Knurled Nuts12/pkg$85order part 132371
132462Lucite Water Bath1/pkg20-Cell Eq. Dialyzer$263order part 132462
1324585-Cell Eq. Dialyzer$210order part 132458
132366Drive Belt5-Cell Eq. Dialyzer$37order part 132366

Click on any part number for pricing information or to place an order.

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