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KrosFlo® Research II TFF System


Hollow Fiber Tangential Flow Filtration System for R&D Volumes (2 ml to 10 liters)

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The KrosFlo Research II TFF System is ideal for performing and monitoring the filtration of R&D scale volumes. Ideal for characterizing a separation or maintaining a validated process, this system combines filtration surveillance with flexibility for a multitude of UF & MF applications. Operated by a 2.3 LPM peristaltic pump, the disposable KrosFlo hollow fiber module quickly and efficiently filters while the disposable flow-path minimizes hold-up volume. The system features the KrosFlo Digital Pressure Monitor that reads & displays process pressures with alarms that can shut-off the pump under high or low pressure conditions. This UL and CE certified system is great for validated processes and protecting valuable samples! The monitor also interfaces with a PC to record all process data into the included KF Comm data collection software. Let the KrosFlo Research II TFF System efficiently filter while protecting your sample.

3 Disposable Flow-Path Types


The entire process flow path which includes pump tubing, connections and 2 reservoirs can be discarded after use along with the disposable HF module. No cleaning, sanitizing or cross-contamination makes validation much easier! There are three system types available depending on your processing volume.

KF Comm Data Collection Software


Interfaces with a computer to download real-time operating pressures, calculated TMP, pump rate, date and triggered alarm points into the specially designed Excel® format software for easy collecting & graphing of process data. Measured flux rates can be manually entered along with the streaming data feed.

KrosFlo Research II TFF System Operating Specifications

Flow-path TypeProcess Vol.Process BottleFeed BottleMin. Hold-upTubing SizeFlow Rate
MicroKros®Up to 60 ml15 ml60 ml5 mlSize 14 (1/16" ID)Up to 130 ml/min
MidiKros®Up to 500 ml125 ml500 ml20 mlSize 16 (1/8" ID)Up to 480 ml/min
MiniKros® Sampler PlusUp to 10 liters500 ml2 liter70 mlSize 17 (1/4" ID)Up to 1.7 L/min

Standard System Components & Features


Designed specifically for application flexibility and process monitoring, the 3 standard KrosFlo Research II TFF Systems models include:
  • KrosFlo Research II Pump 50/60 HZ (110V or 220V)
    • Forward/reverse digital motor control, 10 - 600 RPM
    • Predictive flow for MasterFlex tubing size 13, 14, 16 & 17
    • Programmable calibration, priming & dispensing
    • UL and CE compliant
    • Incorporated flow path stand
    • Pump operating instructions
  • KrosFlo Research II Pump Head with 3 SS Rollers
    • 4 orientations (left, right, up & down)
  • KrosFlo Digital Pressure Monitor (110V or 220V)
    • 3 disposable pressure transducers
    • 4 pressure display outputs:
      • Inlet pressure (w/ 3 alarms & 1 stop)
      • Outlet pressure
      • Permeate pressure (w/ 2 alarms & 1 stop)
      • Transmembrane pressure (calculated)
    • Monitor/pump cable:
      • Pump over-ride control
      • Pump speed (RPM) relay
    • RS-232 Cable for PC Interface
    • Monitor operating instructions
  • 1 of 3 disposable process flow paths
    • MicroKros Module Flow Path
    • MidiKros Module Flow Path
    • MiniKros Sampler Flow Path
  • Feed and process reservoirs
  • KF Comm data collection software
    • Excel format
    • All 4 pressures
    • Pump flow rate
  • System operating instructions

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Advantages of Disposability

  • Easier FDA Validation
  • Saves Money & Time
  • No Cross-Contamination
  • Less Down-Time
  • Low Shear
  • Directly Scalable for Production
  • Easy Retrofit for other HF Modules


Filtration Resources:

Product Literature
Fundamentals of Filtration
Spectrum's Competitive Edge
Our HF Membranes vs. Others
Scalability of Process Modules
Application Guide
Chemical Compatibility Chart
Pore Size Chart



Download Brochure


Research Scale Applications

  • Cell Washing
  • Lysate Clarification
  • Bacterial Concentration
  • MicroParticle Diafiltration
  • Macromolecule Isolation
  • Desalt & Buffer Exchange
  • Viral Clarification
  • Protein Purification
  • Antibody Production
  • Desalt & Buffer Exchange
  • NanoParticle Diafiltration

RELATED PRODUCTS:


MicroKros Hollow Fiber Modules
Hollow Fiber TFF for UF or MF of 2 - 60 ml volumes
Learn More >


MidiKros Hollow Fiber Modules
for volumes of 20 - 500 ml
Learn More >


MiniKros Sampler Hollow Fiber Modules
for volumes of 50 ml - 3 L
Learn More >


MiniKros Sampler Plus Hollow Fiber Modules
for volumes of 50 ml - 3 L
Learn More >

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