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MiniKros® Pilot TFF System


Tangential Flow Filtration (TFF) System for 10 L to 1000 L With Process Controls and Data Acquisition for Lab & Pilot Bioprocessing

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The Spectrum MiniKros Pilot System design allows precise and reproducible control and documentation of processes ranging from 10 to 1000 liters. The system includes a digital 24LPM peristaltic pump, a digital pressure monitor with automated shut-off controls, a permeate flow monitor and real time data collection software. The MiniKros Pilot System is UL and CE certified.


The MiniKros Pilot System was designed to use Disposable KrosFlo® MBT (Module-Bag-Tubing) sets, the fully assembled disposable process flow paths for Tangential Flow Filtration. MBT sets are ideal for upstream TFF microfiltration and downstream TFF ultrafiltration.


Digitally Controlled Peristaltic Pump
Provides precision flow control

  • IP 66 rated stainless steel for easy wash down
  • 650 RPM Pump drive
  • Pumps up to 24LPM with 2 pump heads
  • Reversible motor
  • 115/230 VAC auto select voltage (50/60Hz)
  • Dimensions: 18 3/8" x 11" X 17"
    (467 cm x 279 cm x 432 cm)
  • Shipping weight: 32 kg (70lbs)


Flow Monitor and Meters
Provide a complete module summary for process scale-up and reproducibility

  • Measures & displays feed & permeate flow rates
    • 0.05 - 0.5 LPM and 0.2 - 4.5 LPM
    • +/- 0.1% repeatability
    • +/- 1.5% accuracy
  • USP class 6 Permeate Flow Meters
    • Sterile & Disposable
  • Calibration factor printed on flow meter
  • Calibrated for Masterflex Tubing
    • Numbers 26, 70, 73, 82, 88 or 89
  • Programmable calibration for non standard tubing


Digital Pressure Monitor and Transducers
Interlock to provide complete process control and minimize product loss.

  • Measures & displays 4 pressures
    • Inlet Pressure
    • Outlet Pressure
    • Permeate Pressure
    • Transmembrane Pressure (TMP)
  • 5 user defined pressure alarms set points with pump auto-shutoff option
  • 3 sterile & disposable pressure transducers


KF Comm Data Collection Software
Automatically collects process data and provides real-time overview of the filtration process to save time and insure the reproducibility of separation.

  • Records 8 real-time operating parameters:
    • Time
    • Inlet Pressure
    • Retentate Pressure
    • Permeate Pressure
    • Feed Flow Rate
    • Permeate Flow Rate
    • Pump RPM
    • Alarms
  • Calculates 9 processing parameters:
    • Transmembrane Pressure (TMP)
    • Pressure Drop (DP)
    • Shear Rate
    • Total Permeate Volume
    • Retentate Flow Rate
    • Filtration Flux Rate (LMH)
    • Cross Flow Flux (CFF)
    • Volumetric Throughput (VT)
    • Concentration Factor
  • Automatic graphing of data in Excel based software
  • 21CFR Part 11 enabled when used with optional interface software package

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Advantages of Disposability

  • Easier FDA Validation
  • Saves Money & Time
  • No Cross-Contamination
  • Less Down-Time
  • Low Shear
  • Directly Scalable for Production
  • Easy Retrofit for other HF Modules


Filtration Resources:

Product Literature
Fundamentals of Filtration
Spectrum's Competitive Edge
Our HF Membranes vs. Others
Scalability of Process Modules
Application Guide
Chemical Compatibility Chart
Pore Size Chart



Download Brochure
Download Manual


System Applications:

  • Cell Harvesting/Clarification
  • Virus Purification
  • Cell Perfusion
  • VLP Purification
  • Lysate Clarification
  • Viral Clarification
  • Bacterial Concentration
  • Protein Purification
  • Micro-Particle Diafiltration
  • Antibody Purification
  • Macromolecular Isolation
  • Desalting and Buffer Exchange
  • NanoParticle Diafiltration


RELATED PRODUCTS:

Disposable KrosFlo MBT Sets
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MiniKros Hollow Fiber Modules for volumes 300 ml - 100 L
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MiniKros Plus Hollow Fiber Modules for volumes from 300 ml to 100 L
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CellFlo® Hollow Fiber Modules for volumes from 500 ml - 100 L
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CellFlo Plus Hollow Fiber Modules for volumes from 500 ml - 100 L
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